Taking advantage of the neuron-specific expression of AAV9.hSyn.YFP, we applied AAV9.hSyn.YFP, AAV9.hSyn.YFP-IMP2, and AAV9.hSyn.YFP-HCC in the conditioned culture at 1 DIV and harvested the cells at 4 DIV. This evidence concerns the gene RIC8B and hepatocellular carcinoma.