SPRY2 and colorectal carcinoma: To expand the scope of former studies, we aimed at exploring the abundance of 5mC in 4 putative regulatory regions of SPRY2, based on overlapping H3K27ac and H3K4me3 active chromatin histone markers reflected by ENCODE ChIP-seq data (hg19 source) that are displayed as signal peaks in CRC cell lines Caco2 and HCT116 (Supplementary Figure S2) [34].