LOs are atypical extracellular vesicles, produced as a byproduct of non-apoptotic plasma membrane blebbing from cancer cells, and induced by silencing of the cytoskeletal regulator Diaphanous-related formin-3 (DIAPH3), by overexpression of the oncoproteins MyrAKT1, HB-EGF, and caveolin-1, or by the activation of the EGFR [45,46]. The gene discussed is DIAPH3; the disease is cancer.