AKT1 and colorectal carcinoma: DLD-1 CRC cells were cultured in CM to serve as controls or pretreated with DMSO or LY294002 (Akt inhibitor, 20 μM), or rapamycin (p70S6k inhibitor, 100 nM) for 1 h or pretreated with ad-GFP- or dn-Akt-expressed plasmids for 48 h and then cultured in ACM for 8 h to analyze the SREBP-1 mRNA (real-time PCR) and protein (western blot) expressions or cultured in ACM for 8 h with 5-FU for 24 h to analyze the cell viability (MTT assay).