STAT3 and Familial prostate cancer: In this study, we identified acacetin as a STAT3 inhibitor and verified that acacetin directly binds to STAT3 using various biochemical methods, including a pull-down assay, a drug affinity responsive target stability (DARTS) experiment, and a cellular thermal shift assay (CETSA), in DU145 prostate cancer cells with constitutively active STAT3 [18,19].