These EV proteins consisted of the following: conventional EV markers (CD82, CD81, CD9, CD63, and HSPA8), universal EV markers (CDC42, CNP, FN1, GNAI2, HSPG2, ITGB1, ITGB3, MVP, SLC2A1, TLN1, and TUBA4A), tissue-specific markers (LC-specific: EPS15, GPRC5A, and TSG101), and adenocarcinoma-cell-line-specific markers (A549 cell line: ITGA3, PACSIN2, PDCD6IP, PTGFRN, and RACGAP1; NCI-H23 cell-line-specific markers: ICAM1, MFGE8, and SDC4). The gene discussed is FN1; the disease is adenocarcinoma.