Based on the above, this study used sodium oleate to induce NRK-52e cells to detect the levels of EMT and fibrosis-related proteins and found that the expression of α-SMA, Snail1, ZEB1, and TGF-β1 were extremely increased, and the expression of CD31 was extremely reduced, indicating that NRK-52e cells appear in EMT and fibrosis similar to diabetic nephropathy. This evidence concerns the gene ZEB1 and diabetic kidney disease.