Similarly, a placebo-controlled clinical trial, including 65 overweight or obese adults with vitamin D deficiency (25(OH)D concentration < 20 ng/mL) randomly divided into supplemented (oral bolus dose of 100,000 IU cholecalciferol followed by 4000 IU cholecalciferol/day) and placebo groups for 16 weeks, found no improvements in insulin resistance or secretion parameters associated with vitamin D supplementation [77]. The gene discussed is INS; the disease is vitamin D deficiency.