In accordance with the associations of circulating C22:0 and C24:0 with parameters of metabolic dysfunction observed in human studies, mice fed on a high-fat diet exhibit decreased hepatic expression of CerS2 and decreased levels of ceramides containing C22:0 and C24:0 while CerS2-null mice develop insulin resistance and impaired hepatic insulin signaling [40,41]. This evidence concerns the gene INS and Insulin resistance.