CLDN4 and infection: To further emphasize the location of CLDN-4, black-and-white images of CLDN-4 expression in MOCK- and Ct-infected pOECs from a representative pOEC line are shown in Figure 7B. These images demonstrate that while the CLDN-4 expression in MOCK-infected pOECs stays at the cell membrane, CLDN-4 in Ct-infected pOECs is present on surface only at the beginning (0 and 6 hpi) and at the end (48 and 72 hpi) of the Ct infection cycle.