We additionally use these relevant primary pOECs to demonstrate that while the overall mRNA and protein expression of CLDN-4 is unaltered (Figure 5 and Figure 6, respectively), Ct infection triggers a redirection of CLDN-4 into the Ct inclusion that leads to a decrease in CLDN-4 expression on the pOEC membrane (Figure 7). The gene discussed is CLDN4; the disease is infection.