Spearman’s rank correlation coefficient correlation calculation showed statistically significant correlations between the number of immunopositive structures for PAX7, PAX9, and RYK within the epithelium and the connective tissue within each type of cleft tissue analyzed in the study (unilateral cleft lip patient group, bilateral cleft lip patient group, and isolated cleft palate patient group). The gene discussed is PAX9; the disease is cleft palate.