With a coating of anti-CD44v6 single-chain variable fragment for targeted delivery, the miR-21 antisense oligonucleotide and gemcitabine dual-treatment nanoparticle up-regulated programed cell death protein 4 and phosphatase and tensin homolog (PTEN) expression, thus suppressing epithelial-mesenchymal transition, cell proliferation, migration, and invasion of pancreatic cancer cells. This evidence concerns the gene PTEN and familial pancreatic carcinoma.