Whereas in U-87 MG GBM cells, an impact of PIM1 inhibitors was absent, LN-18 cells showed a strong down-regulation of Nestin protein after treatment with either LY294002, QT or TCS, particularly at a concentration of 50 μM, from a relative value of 1.0 (±0.06, control) to 0.56 (±0.22, LY), 0.40 (±0.28, QT) and 0.29 (±0.11, TCS), respectively. Here, PIM1 is linked to glioblastoma.