To investigate whether inhibition of PIM1 also leads to cytotoxic effects in stem-like LN-18 neurospheres, we treated LN-18 neurospheres with TCS (specific PIM1 inhibitor) [35] and LY294002 (dual PI3K/PIM1 inhibitor) [33] and compared the effects with temozolomide as the standard therapeutic agent in GBM. The gene discussed is PIM1; the disease is glioblastoma.