In the present study, we showed that single intrathecal administration of the two highest doses of CXCR2 (NVP CXCR2 20) and CXCR3 ((±)-NBI 74330) antagonists to rats resulted in a strong, fast (observed already after one hour), and persistent analgesic effect for up to 6 h on day 7 after peripheral nerve injury, when neuropathic pain was fully developed. The gene discussed is CXCR2; the disease is peripheral nerve injury.