TLR4 and cholestasis: With respect to a 28-day exposure to PQ 2 μM, only minor changes in the expression of genes related to drug-induced cholestasis could be observed in one or two donors, including a few genes related to inflammation (i.e., colony stimulating factor, serpin E1 and toll like receptor 3), apoptosis (i.e., lymphocyte antigen 96 and toll like receptor 4), autophagy (i.e., sequestosome 1) and necroptosis (i.e., MLKL) (Figure 4b).