In this regard, the downregulation of CYP7A1, organic anion transporter 1B1 and SLC10A1 in two donors is compliant with the AOP on drug-induced cholestasis [17] and confirms results previously obtained in spheroid cultures of HepaRG cells and in PHH in long-term 2D-sandwich culture [18,20]. The gene discussed is CYP7A1; the disease is cholestasis.