To identify the mechanism of the MSPA-induced cytotoxic effect on cancer cells, HepG2 and HL60 cells were treated with 12.5 μM of MSPA for 72 h and analyzed via flow cytometry after double staining the cells with Annexin V/7-amino-actinomycin D (7-AAD) (Figure 1B). The gene discussed is ANXA5; the disease is cancer.