In a previous study from our laboratory [39] EMT was induced in A549 lung adenocarcinoma cells, ACHN kidney adenocarcinoma cells derived from metastatic pleural sites, and MCF10A immortalized breast cells using a combination of TGF-β and TNF-α, and gene expression was analyzed using microarray protocols. The gene discussed is TGFB1; the disease is renal cell adenocarcinoma.