EGFR and cystitis: Similarly, EGFR transcriptional levels were assessed, through real-time PCR, between healthy dogs (n = 3) and dogs with MIUC (n = 4) while protein translation was assessed through immunohistochemistry (IHC), between dogs with MIUC (n = 25), dogs with polypoid cystitis (n = 5) and healthy dogs (n = 5).