TFEB and neoplasm: Although TFE3 (and TFEB) immunohistochemistry may be practical screening techniques for diagnosing MiT translocated RCCs in daily routine (with only strong, diffuse positivity in tumor cells being interpreted as positive, since physiological low levels of TFE3 may also be encountered in tumors and adjacent stromal cells), the gold-standard approach remains documenting specific translocations with break-apart FISH, especially because it is less susceptible to fixation issues in paraffin-embedded samples [70].