Even if the trend to lower methylation levels of PBX1 and RASA3 did not reach statistical significance, probably due to the limited number of samples, we demonstrate the significant hypomethylation of FLJ43663 also in cfDNA from TNBC patients compared to plasma from healthy women, mirroring the epigenetic alteration found in the tumoural tissue. This evidence concerns the gene RASA3 and neoplasm.