PPARG and obesity due to melanocortin 4 receptor deficiency: Motif enrichment analysis showed that genomic regions with H3K4me3 modification were enriched for binding sequences of known adipose regulators such as PPARG known to be the key factor in adipogenesis [15,16] and required for adipocyte identity and survival of mature adipocytes (loss of PPARG activity is related to the acquisition of a fibroblast-like phenotype in adipocytes during obesity); EBF1 (a direct target of PPARG) and EBF2 are transcription factors necessary for adipogenesis of 3T3-L1 cells [17].