Further, the Western blot result showed that the protein expression of BNP (Figure 7C), an indicator of pressure overload/heart failure biomarker, and the protein expression of ß-MHC (Figure 7D), an indicator of cardiomyocyte hypertrophy, were lowest in group 1 and significantly progressively reduced from groups 2 to 5. This evidence concerns the gene NPPB and heart failure.