Within this framework, the present study aimed at investigating the anticancer properties of 3 kDa-fraction of whey in CRC cell lines focusing on its effects as regulator of SIRT3, mitochondrial metabolic pathways, SIRT3-related metabolic targets regulating the energy metabolism, such as PPAR, LDHA and SREBP, and mitochondrial oxidative metabolism. This evidence concerns the gene SIRT3 and colorectal carcinoma.