Additional work has also demonstrated, in in vivo tumour models, that cyroablation of melanoma resulted in the expansion of ALCAM-positive cell populations, which were comprised of CD45 − mesenchymal stem/stromal cells, CD11b +Gr1 + myeloid-derived suppressor cells, and CD4 +Foxp3 + regulatory T cells [143]. The gene discussed is ALCAM; the disease is melanoma.