Based on the EpCAM-independent subtraction enrichment (SE) and immunostaining-fluorescence in situ hybridization (SE-iFISH), which is suitable for processing specimens of both patients’ and patient-derived xenograft (PDX) tumor mouse models [19,22,23], a novel strategy to distinguish aneuploid necrotic cells (NC) from viable cancer cells was developed in this study. Here, EPCAM is linked to neoplasm.