Beyond our previous SE-iFISH technology, which was quantitatively validated on spiked cancer cells and clinical specimens [22,43], comprehensive identification and characterization of the enriched live and dead cancer cells performed by the innovated iFISH (NC) upon cell size [24,44], stemness [38] and tumor marker expression as well as aneuploid chromosomes [9,10,22,30], is demonstrated in the present study, showing a necrotic triploid NSCLC cell expressing EMT-related EpCAM and vimentin (the hybrid E/M phenotype) (Figure 1C). This evidence concerns the gene EPCAM and neoplasm.