In vivo, after orthotopic implantation of tumor tissue in nude mice, serum AFP levels were 246 ± 66 μg/L for MHCC97-H with high metastatic potential and 91 ± 66 μg/L for MHCC97-L with low metastatic potential (p < 0.01, t test) [111]. This evidence concerns the gene AFP and neoplasm.