Validated down-regulated genes include NPC2 involved in the negative regulation of MAPK [38], TSPAN8 involved in tumor progression and metastasis [39], ANKRD1 mainly expressed in MDA-MB231 breast cancer cell lines and linked to treatment-resistance in breast cancer [40,41], and FST involved in metastasis suppression [42] (Figure S2D). This evidence concerns the gene TSPAN8 and breast cancer.