IQGAP1 and non-small cell lung carcinoma: We performed immunoprecipitation assays to detect CRMP2 and IQGAP1 interaction using protein extracts from migrating A549 NSCLC cells that confirmed the absence of CRMP2-IQGAP1 interaction in CRMP2 phosphorylation-mimetic (S522D) mutants (Figure 7C).