Its role in the calcification of extraosseous cell/tissues is demonstrated in a mouse model of atherosclerosis, where Runx2 is upregulated and colocalized in calcified atherosclerotic lesions [64], and mice with SMC-specific Runx2 deficiency have normal aortic anatomy but have lower high-fat diet-induced vascular calcification [65]. This evidence concerns the gene RUNX2 and atherosclerosis.