The aims of the present study were: (1) to evaluate the expression and distribution pattern of TFF1 in normal gastric mucosa, GPs, and malignant gastric tumors with neoplastic emboli and corresponding metastasis in dogs; (2) to determine whether there was an association between the expression of this protein and clinicopathological features of the tumors and patient’s survival time. Here, TFF1 is linked to gastric cancer.