Another study using the rat MCAO model revealed that Nec-1 reduced infract volume and attenuated ischemia-related neurological deficits, and these effects were accompanied by a decrease in necroptotic (p-RIP1+neurons, p-RIP1, RIP3, MLKL, pMLKL) and inflammatory (IL-1β) markers [128]. The gene discussed is RIPK1; the disease is ischemia.