In this study, we observed that a non-toxic concentration of 5 μM of 5-AZA reduced significantly the expression of DNMT1 protein in non-tumoral cells IHH and stromal subtypes HLE, HLF, and JHH6, but not that of CSC subtypes HepG2 and Huh7, even though DNMT1 basal expression in HCC lines was comparable. This evidence concerns the gene DNMT1 and hepatocellular carcinoma.