Lastly, zonulin, which is a tight junction protein linked to intestinal barrier integrity, was found to be increased in intestinal biopsies of IBD dogs treated with probiotics (plus standard therapy, including prednisone), compared to controls [40], when evaluated by immunohistochemistry in intestinal biopsies; however, it also decreased after therapy (prednisone and diet) in canine IBD, as well as in intestinal biopsies [41]. The gene discussed is HP; the disease is irritable bowel syndrome.