In particular, it was shown that hepatic transcriptional activity of aminotransferases (AST and ALT) was significantly upregulated in NAFLD patients and alanine was correlated with hepatic mRNA expression of GPT (gene encoding the cytosolic isoform ALT1), GPT2 (gene encoding the mitochondrial isoform ALT2), and GOT1 (gene encoding the cytoplasmic GOT). Here, GPT2 is linked to metabolic dysfunction-associated steatotic liver disease.