EGFR and neoplasm: The EFIRM-based method was also used in the detection of the prognostic biomarker in liquid biopsy such as ultra-short circulating tumour DNA (usctDNA) in plasma and saliva [87] and p.L858R EGFR, exon19 del EGFR, p.E545K PIK3CA ctDNA in plasma [88] and exosomes [43].