To profile the role of specific MT isoforms and to identify promising biomarkers for HCC, here we evaluated the mRNA expression level of MTs between HCC and normal liver tissues via Oncomine and GEPIA databases and analyzed their prognostic value using the Kaplan–Meier plotter database, which were well validated in GSE54236, GSE116174, and ICGC-LIRI-JP cohorts. This evidence concerns the gene MCAT and hepatocellular carcinoma.