We maintained the treatment schedule and continued to monitor tumor growth until mouse tumor volume surpassed the ethical limit (2000 mm3 or approximately 10% of body weight), at which point mice were sacrificed and a portion of resected HCT-116 p53−/− and HCT-116 p53+/+ tumors was fixed in formalin and embedded in paraffin. The gene discussed is TP53; the disease is neoplasm.