IFNG and cyst: As expected, IFN-γ levels decreased dramatically in all groups 15days after the challenge: ~3,500pg/ml for plAtHsp81.2-SAG1HC vs. ~1,400, ~1,100, and ~1,800pg/ml for PBS, control, and rAtHsp81.2-SAG1HC groups, respectively, which is consistent with the establishment of the chronic infection phase and parasitic cyst formation (Figure 7C).