AFP and hepatocellular carcinoma: reported that after combined treatment of primary HCC patients with CIK cells and local radiofrequency (RF) hyperthermia, T and NKT cells increased significantly, and alpha-fetoprotein (AFP) decreased from 167.67 ± 22.44 to 99.89 ± 22.05 ng/ml (P = 0.001) (55).