To further explore the function of Tim-1 in diet-induced hepatic steatosis, Tim-1-/- mice on C57BL/6J background were generated by inserting a Lox-Stop-Lox cassette into intron 2 of Tim-1 using CRISPR/Cas9 technology (Figures 2A, B). Here, HAVCR1 is linked to fatty liver disease.