To determine the influence of sodium butyrate on the TLR4/NF-κB intestinal signaling pathway in NEC mice, we assessed TLR4 and NF-κB mRNA expression by QPCR and TLR4 and NF-κB protein expression by western blotting and immunohistochemistry. The gene discussed is NFKB1; the disease is necrotizing enterocolitis.