In order to determine whether HSV-1 infection resulted in a reorganization of these components, we first confirmed the infection efficiency of HSV-1 in HeLa cells (Supplementary Fig. 1a) and C-33A cells (Supplementary Fig 1b), and then performed an RNA fluorescence in situ hybridization (FISH) and immunofluorescence assay to examine the interaction between NEAT1, PSPC1, P54nrb, and SRSF2 in HSV-1 or Mock infected HeLa and C-33A cells. This evidence concerns the gene PSPC1 and infection.