The METABRIC database (1904 human breast tumors) consistently revealed a positive association of NME4 with epithelial markers CDH1 and KRT18 (Additional file 22: Fig. S15A) and a negative association with mesenchymal markers CDH2 and VIM (Additional file 22: Fig. S15B) as well as many EMT drivers like ZEB1, ZEB2, SNAI2, TWIST1, and TWIST2 (Additional file 22: Fig. S15C-E). This evidence concerns the gene TWIST1 and breast neoplasm.