By 3.5 days post-infection with the GFAP-Math5-Brn3b-tdTomato AAVs, no tdTomato+ axons were seen in the optic nerve (Supplementary Figure 5F), in agreement with the observation that there were no RGCs reprogrammed from MG by this time (Figure 1E). The gene discussed is GFAP; the disease is infection.