We observed SPRY4-AS1 expression was not associated with age (p = 0.079; Figure 2A), However, we observed that the expression of SPRY4-AS1 in HCC was distinctly linked to gender (p = 0.00016; Figure 2B), grade (Figure 2C), stage (Figure 2D), and cancer status (p = 0.0039; Figure 2E). This evidence concerns the gene SPRY4 and hepatocellular carcinoma.