Our study found that rs2234693, rs9340799, and rs4986938 polymorphisms, the most extensively investigated in ESR1 and ESR2, might not play an essential role in PCOS susceptibility in the allele model, the dominant model, the recessive model, the heterozygote model, or the homozygote model from pooled analysis. This evidence concerns the gene ESR2 and polycystic ovary syndrome.