At E18.5, all Dyrk2−/− embryos displayed multiple defects, including the omphalocele phenotype, craniofacial development, short limb, and anal atresia, as well as an open eyelid phenotype at times (Fig. 1a–c and Supplementary Fig. 4a–c). The gene discussed is DYRK2; the disease is imperforate anus.