Real-time PCR analysis of stemness-related markers revealed that mRNA levels of ABCG2, C-MYC, CD133, NANOG, and KLF4 were significantly upregulated in GOLPH3-overexpressing cells, while the levels were downregulated in GOLPH3-silenced NSCLC cells. The gene discussed is NANOG; the disease is non-small cell lung carcinoma.