Interestingly at 24 h, there was an almost five-fold increase in IL8 (i.e. following exposure to ZnO NMs at 25 μg/ml—steatosis MT 83 ± 12.9 pg/ml vs. NASH MT 506.3 ± 9.82 pg/ml) and almost ten-fold increase in IL6 (i.e., following exposure to TiO2 NMs at 25 μg/ml—steatosis MT 11.84 ± 1.17 pg/ml vs. NASH MT 196.2 ± 13.5 pg/ml) in NM-induced cytokine levels in the NASH MT as compared to the steatosis MT. This evidence concerns the gene IL6 and metabolic dysfunction-associated steatohepatitis.