Interestingly in a similar pattern to above, a concentration-dependant increase in IL6 and IL8 was also observed in the diseased MT following exposure to isoniazid with the inflammatory response significantly higher for the NASH model compared to the steatosis tissue (i.e., 24 h 100 mM isoniazid exposure—IL6—healthy MT 25 ± 3.24 pg/ml, steatosis MT 74.4 ± 13.4 pg/ml vs. NASH MT 295.9 ± 10.1 pg/ml; IL8—healthy MT 240.9 ± 37.1, steatosis MT 418.5 ± 19.1 pg/ml vs. NASH MT 502.5 ± 34.9 pg/ml) (Fig. 6 and Supplementary Fig. 9). Here, CXCL8 is linked to metabolic dysfunction-associated steatohepatitis.