CASP3 and neoplasm: Immunohistochemical (IHC) staining also showed that tumor sections from nude mice that injected subcutaneously with TM7SF2-KO C33A cells expressed more apoptotic marker Cleaved caspase-3, while TM7SF2-OE C33A cells showed lower expression of Cleaved caspase-3 (Fig. 7C).